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hiscript ii reverse transcriptase  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript ii reverse transcriptase
    Hiscript Ii Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+ii+reverse+transcriptase/HiScript+II+Reverse+Transcriptase/custom%40r201%4042495521
    Average 97 stars, based on 1466 article reviews
    hiscript ii reverse transcriptase - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Synthesized:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Article Title: An oncolytic vaccinia virus encoding CD47 nanobody potentiates antitumor immunity in multiple myeloma
    Article Snippet: To assess viral biodistribution, viral DNA was extracted from 25 mg of tissue homogenate using the Viral DNA/RNA Kit (Cat# CW0548S, Cowin Biotech). .. To evaluate αCD47nb expression, total RNA was isolated with the FastPure Cell/Tissue Total RNA Isolation Kit (Cat# RC101–01, Vazyme Biotech). cDNA was synthesized from 1 μg of RNA using HiScript II Reverse Transcriptase (Cat# R201–01, Vazyme Biotech). ..

    Reverse Transcription:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    Article Title: An oncolytic vaccinia virus encoding CD47 nanobody potentiates antitumor immunity in multiple myeloma
    Article Snippet: To assess viral biodistribution, viral DNA was extracted from 25 mg of tissue homogenate using the Viral DNA/RNA Kit (Cat# CW0548S, Cowin Biotech). .. To evaluate αCD47nb expression, total RNA was isolated with the FastPure Cell/Tissue Total RNA Isolation Kit (Cat# RC101–01, Vazyme Biotech). cDNA was synthesized from 1 μg of RNA using HiScript II Reverse Transcriptase (Cat# R201–01, Vazyme Biotech). ..

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..

    Article Title: CircNOLC1/KRT16 promotes colorectal cancer invasion and migration via c-met nuclear translocation and reprogramming of the oxidative pentose phosphate pathway
    Article Snippet: .. Total RNA was extracted from cells using the Trizol reagent kit (Accurate Biology), and reverse transcription was performed using HiScript II reverse transcriptase (Vazyme Biotech). ..

    Article Title: The Transcription Factor SOX6 Regulates Autophagy Levels Through LAMP5 to Inhibit the Development of Pituitary Adenomas.
    Article Snippet: Pituitary adenoma (PA) is a common type of brain tumor, and existing treatment options have limited efficacy in clinical settings.. Autophagy is a risk factor for PA progression, and lysosome-associated membrane protein family member 5 (LAMP5) plays a regulatory role in tumor autophagy.. Therefore, this study aimed to investigate the role of LAMP5 in PA autophagy, laying the foundation for the development of alternative therapies for PA. A tumor-bearing model was constructed by injecting rat pituitary tumor cells (GH3) into the left back of nude mice.

    Quantitative RT-PCR:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    SYBR Green Assay:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Control:

    Article Title: Ultrasound-activated piezoelectric hydrogel with schwann cell-derived extracellular vesicles enhances neural cell activities: An in vitro study.
    Article Snippet: Peripheral nerve injury (PNI) often leads to incomplete functional recovery because the regulation of the regenerative microenvironment is insufficient.. To address this issue, we developed a photopolymerized composite hydrogel (GelMA-SC-EVs-BTO, GSB), which integrates barium titanate (BaTiO3, BTO) nanoparticles and Schwann cell-derived extracellular vesicles (SC-EVs) into the GelMA matrix, aiming to provide synergistic ultrasound-activated piezoelectric stimulation and continuous EV-mediated biological activity.. Physical and chemical property analysis confirmed its porous structure, uniform barium/titanium distribution, stable piezoelectric response (d33 increase, repeatable on/off current output), and continuous release of SC-EVs.

    Isolation:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    Article Title: An oncolytic vaccinia virus encoding CD47 nanobody potentiates antitumor immunity in multiple myeloma
    Article Snippet: To assess viral biodistribution, viral DNA was extracted from 25 mg of tissue homogenate using the Viral DNA/RNA Kit (Cat# CW0548S, Cowin Biotech). .. To evaluate αCD47nb expression, total RNA was isolated with the FastPure Cell/Tissue Total RNA Isolation Kit (Cat# RC101–01, Vazyme Biotech). cDNA was synthesized from 1 μg of RNA using HiScript II Reverse Transcriptase (Cat# R201–01, Vazyme Biotech). ..

    RNA Extraction:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    cDNA Synthesis:

    Article Title: Lactic acid promotes metastasis of papillary thyroid carcinoma by enhancing CPT1A lactylation.
    Article Snippet: .. Total RNA was isolated using an RNA extraction kit (ESScience, RN001). cDNA synthesis was performed with HiScript II Reverse Transcriptase (Vazyme, RL201-01). .. RT-qPCR was conducted using ChamQ SYBR Master Mix (Vazyme, Q711-02) on a Bio-Rad CFX96 system.

    Expressing:

    Article Title: An oncolytic vaccinia virus encoding CD47 nanobody potentiates antitumor immunity in multiple myeloma
    Article Snippet: To assess viral biodistribution, viral DNA was extracted from 25 mg of tissue homogenate using the Viral DNA/RNA Kit (Cat# CW0548S, Cowin Biotech). .. To evaluate αCD47nb expression, total RNA was isolated with the FastPure Cell/Tissue Total RNA Isolation Kit (Cat# RC101–01, Vazyme Biotech). cDNA was synthesized from 1 μg of RNA using HiScript II Reverse Transcriptase (Cat# R201–01, Vazyme Biotech). ..

    Polymerase Chain Reaction:

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..

    Real-time Polymerase Chain Reaction:

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: Total RNA was extracted using a Bacterial RNA Kit (Omega Bio-tek). .. For quantitative reverse transcription PCR (RT-qPCR), 1 μg of RNA was converted to complementary DNA (cDNA) using HiScript II Reverse Transcriptase (Vazyme) in 20 μl reactions. qPCR was performed using the aforementioned method with 0.5 ng of cDNA as a template. gapA was used for normalization. ..



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